Synthesis of hydrophobic peptides: An Fmoc “Solubilising Tail” method

摘要

The development of an Fmoc method for synthesis and purification of hydrophobic peptides using a “solubihsing tail” strategy is described. Peptide-constructs of the form hydrophobic peptide-[CHmb ester]-solubilising peptide were synthesised. Procedures for forming the 4-Hmb ester linkage, and sequences of solubilising peptides suitable for use with Fmoc SPPS, were investigated. The method was used to synthesise two model peptides and three designed transmembrane peptides.