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Dual-laboratory validation of a method for the determination of fructans in infant formula and adult nutritionals.

机译:用于确定婴儿配方食品和成人营养食品中果聚糖的方法的双实验室验证。

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摘要

Until recently, only two AOAC Official Methods SM have been available for the analysis of fructans: Method 997.08 and Method 999.03. Both are based on the analysis of the fructan component monosaccharides (glucose and fructose) after hydrolysis. The two methods have some limitations due to the strategies used for removing background interferences (such as from sucrose, α-glucooligosaccharides, and free sugars). The method described in this paper has been developed to overcome those limitations. The method is largely based on Method 999.03 and uses combined enzymatic and SPE steps to remove the interfering components without impacting the final analytical result. The method has been validated in two laboratories on infant formula and adult nutritionals. Recoveries were in the range of 86-119%, with most being in the range of 91-104%. RSDr values were in the range of 0.7-2.6%, with one exception when the fructan concentration was close to the LOQ, resulting in an RSDr of 8.9%. The performance is generally within the requirements outlined in the AOAC Standard Method Performance Requirements (SMPR® 2014.002), which specifies recoveries in the range of 90-110% and RSDr values below 6%.
机译:直到最近,只有两种AOAC官方方法SM可用于果聚糖的分析:方法997.08和方法999.03。两者均基于水解后果聚糖成分单糖(葡萄糖和果糖)的分析。由于用于消除背景干扰(例如来自蔗糖,α-葡萄糖寡糖和游离糖的干扰)的策略,这两种方法有一些局限性。本文中描述的方法已被开发来克服这些限制。该方法主要基于方法999.03,并使用组合的酶促步骤和SPE步骤去除干扰组分,而不会影响最终分析结果。该方法已在两个婴儿配方奶粉和成人营养品实验室中得到验证。回收率在86-119%的范围内,大多数在91-104%的范围内。 RSDr值在0.7-2.6%的范围内,其中果聚糖浓度接近LOQ时例外,导致RSDr为8.9%。该性能通常在AOAC标准方法性能要求(SMPR®2014.002)中概述的要求之内,该要求指定了90-110%的回收率和低于6%的RSDr值。

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