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Nitrolancea hollandica gen. nov., sp. nov., a chemolithoautotrophic nitrite-oxidizing bacterium isolated from a bioreactor belonging to the phylum Chloroflexi

机译:荷兰硝化枪十一月,sp。 nov。,从属于叶绿屈菌门的生物反应器中分离出的一种自化自养亚硝酸盐氧化细菌

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摘要

A novel nitrite-oxidizing bacterium (NOB), strain LbT, was isolated from a nitrifying bioreactor with a high loading of ammonium bicarbonate in a mineral medium with nitrite as the energy source. The cells were oval (lancet-shaped) rods with pointed edges, non-motile, Gram-positive (by staining and from the cell wall structure) and non-spore-forming. Strain LbT was an obligately aerobic, chemolitoautotrophic NOB, utilizing nitrite or formate as the energy source and CO2 as the carbon source. Ammonium served as the only source of assimilated nitrogen. Growth with nitrite was optimal at pH 6.8-7.5 and at 40 °C (maximum 46 °C). The membrane lipids consisted of C20 alkyl 1,2-diols with the dominant fatty acids being 10MeC18 and C18 : 1ω9. The peptidoglycan lacked meso-DAP but contained ornithine and lysine. The dominant lipoquinone was MK-8. Phylogenetic analyses of the 16s rRNA gene sequence placed strain LbT into the class Thermomicrobia of the phylum Chloroflexi with Sphaerobacter thermophilus as the closest relative. On the basis of physiological and phylogenetic data, it is proposed that strain LbT represents a novel species of a new genus, with the suggested name Nitrolancea hollandica gen. nov., sp. nov. The type strain of the type species is LbT ( = DSM 23161T = UNIQEM U798T).
机译:在以亚硝酸盐为能源的矿物培养基中,从硝化生物反应器中分离出一种新型的亚硝酸盐氧化细菌(NOB)LbT菌株。细胞为椭圆形(刺血针形)杆,具有尖锐的边缘,不运动,革兰氏阳性(通过染色和来自细胞壁结构)和无孢子形成。 LbT菌株是专性需氧的,化学自养型NOB,利用亚硝酸盐或甲酸作为能源,CO2作为碳源。铵是吸收氮的唯一来源。在pH 6.8-7.5和40°C(最高46°C)下,亚硝酸盐的生长最佳。膜脂由C20烷基1,2-二醇组成,主要脂肪酸为10MeC18和C18:1ω9。肽聚糖缺乏meso-DAP,但含有鸟氨酸和赖氨酸。主要的脂醌是MK-8。对16s rRNA基因序列进行系统进化分析,将LbT菌株置于嗜热链球菌门上的嗜热弯曲杆菌类嗜热菌中,以最接近的亲缘细菌为嗜热链球菌。根据生理和系统发育数据,建议菌株LbT代表新属的一个新种,建议名称为Nitrolancea hollandica gen。十一月,sp。十一月类型种类的类型菌株为LbT(= DSM 23161T = UUNIQEM U798T)。

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