Use of multiple media for the cultivation of mycobacteriaudfrom specimens other than sputum

摘要

An investigation was carried out on the efficacy of using multiple media in the isolationudof Mycobacterium tuberculosis from specimens other than sputum, during theudperiod 1980-1984. Of a total of 3807 specimens examined, 818 were urine, 1428udcerebrospinal fluid (CSF), 567 lymph glands, 94 pus samples, 224 operation specimens,ud91 gastric aspirates, 108 ascitic fluid and 477 were other types of specimens.udEach specimen was inoculated onto one set of media consisting of two slopes each ofud‘Lowenstein-Jensen’ medium (LJ), LJ medium containing 0.5 per cent sodiumudpyruvate (LJP) and 7H11 oleic acid albumin medim (7H11) and two bottles ofudselective Kirchner’s liquid medium (KL). In all, 550 (14%) were positive by cultureudin any one of the four media used. Considering the different media individually,udKL had the highest efficiency yielding 339 (62%) of the total 550 positive cultures.udAlso, KL detected 162 positives which were not positive by any of the other media.udThis was followed by LJ with 328 (60%) positives. LJ and KL when consideredudtogether detected 93 per cent of the positives and LJ, LJP and KL increased theudpositivity to 99 per cent. Thus LJ and KL seems to be the best combination for theudisolation of mycobacteria from specimens other than sputum.