A validated high-performance liquid chromatography methodudfor the determination of rifampicin and desacetyl rifampicin inudplasma and urine

摘要

Objective: To standardize a high-performance liquid chromatography (HPLC) method for the determinationudof rifampicin (RMP) and its major metabolite desacetyl rifampicin (DRMP) in plasma andudurine.udMaterial and Methods: A simple, specific and sensitive HPLC method was developed for the determinationudof RMP and DRMP in plasma and urine. Separation in both was achieved by reversephaseudchromatography on a C18 column with a mobile phase composition of 0.05 M phosphateudbuffer: acetonitrile (55:45 v/v) at 254 nm.udResults: The retention times of DRMP, RMP and Rifapentine (RPN), the internal standard were 2.9,ud4.8 and 10.5 min respectively. The assay was linear from 0.25 to 15.0 μg ml-1 for plasma and 2.5 toud80.0 μg ml-1 for urine. Both intra-day and inter-day accuracy and precision data showed goodudreproducibility.udConclusion: The HPLC method described is sensitive, selective and linear for the wide range ofudconcentrations for RMP and DRMP in plasma and urine. Thus, the method developed is well suitedudfor the pharmacokinetic studies.