Development and applications of quaternary ammonium (QA) membrane electrodes in pharmaceutical preparation and in bioavailability of Prostaglandin E1 and Deoxycholate

摘要

The two novel ion-pairs (PB-TPB and NB-TPB) of quaternary ammonium drugs; propantheline bromide (PB), N,N-Diisopropyl-N-methyl-N-[2-(xanthen-9ylcarbonyloxy)ethyl] ammonium bromide and neostigmine bromide (NB), 3-(dimethylcarbamoyloxy) phenyl]-trimethylazanium have been synthesized, respectively and incorporated in poly (vinyl chloride)-based membrane electrodes for the quantification of propantheline bromide and neostigmine bromide in different pharmaceutical preparations. The influences ofmembranecompositionsonthepotentiometric responses ofmembrane electrodes have been found to substantially improve the performance characteristics. The best performance was reported with membranes having composition (w/w) of PB-TPB or NB-TPB (6%): PVC (34%): o-NPOE (60%). The proposed electrodes exhibit nernstian response in the concentration ranges of 2.1×10−7 M to 1.0×10−2Mand 4.4×10−7 Mto1.0×10−2Mwith detection limit of 1.5×10−7Mand 3.3×10−7 M, respectively. Both the membrane electrodes perform satisfactorily over pH ranges of (3.5–7.5 and 4.0–7.0) with fast response times (11 s and 13 s), respectively. These drugs (PB and NB)were further utilized as different ion-pairs of Prostaglandin E1 (PGE1) and Deoxycholate (DOC) in poly (vinyl chloride)-based membrane electrodes for the determination of bioavailability of Prostaglandin E1 and Deoxycholate in plasma of different patients.