Učinak pentadekapeptida BPC 157 na stvaranje ugrušaka u aortnoj anastomozi u štakora Effect of pentadecapeptide BPC 157 on clot formation at aortal anastomosis in rats

摘要

Diseases that are the result of large vessels thrombosis are a subject of numerous scientific research programmes because of their clinical and economic aspect, but the thrombosis also happens in microcirculation. Microvascular thrombi are found in number of diseases and conditions like thrombocytopenic plethora, sepsis, disseminated intravascular coagulation etc. The significance of thrombosis and the importance of its prevention represent an important medical and economic goal. udIn this paper, we focused on impact of BPC 157 (in which we already proved citoprotectiv effect) on clot formation at the site of microvascular endothel injury cause by operation. Abdominal aorta was transected between renal and illiac arteries, and then anastomosised with Prolen 8/0 or 9/0 sutures. We operated a 100 animals, of which 50 received BPC 157 or saline right after the procedure, and the other half the same but after 24 hours. All the groups were measured the lower extremities strength, before and after procedure, and we did the walk analysis with the specific test. Histology specimens were harvested from all animals 24 hours after the surgery. udAll groups of animals that received BPC 157, weather it was microgram or nanogram concentration, or whether it was administered locally or by mouth, showed that there was no lot present in the histological aorta specimens. That result was also confirmed in functional test and walk analysis, where we noticed that the strength of the lower extremities was not impaired, compared to the values taken before the operation. udOn the other hand, control groups had statistically significantly different results. The ability to walk and lower extremities strength was significantly diminished after the surgery, and on histology specimens there was a clot in the aortal lumen. udWe showed that BPC 157 has a strong effect on clot prevention, when there is an endothel injury (transection and anastomosis), but most importantly his effect on already formed clot in animals who received BPC 157 24 hours after surgery.