Poremećaji genskoga izražaja u bolesnika s juvenilnim seronegativnim spondiloartropatijama Distinctive gene expression in patients with juvenile seronegative spondyloarthtopathy

摘要

Introduction: Juvenile spondyloarthritis (jSpA) refers to spectrum of unpredictableudinflammatory diseases with overlapping features in children younger than 16 years. There is audstrong association of jSpA with HLA-B27 genotype, but many studies shows that otherudgenetic factors, of which we have little or no knowledge, plays a role in the development ofudjSpA. ----- Patients and methods: In this study participated 45 children with obtained HLA genotypeudand diagnosis of jSpA according to ILAR criteria, as well as 11 children with other forms ofudJIA and 12 children without diagnosis of inflammatory disease. DNA microarray geneudexpression was preformed in 11 patients with jSpA and in 4 participants without diagnosis ofudinflammatory disease, along with bioinformatic analysis of retrieved data. Carefully selecteduddifferentially expressed genes where analyzed by qRT-PCR in all participants. ----- Results: Microarray results and bioinformatic analysis revealed 745 differentially expressedudgenes involved in various inflammatory processes in jSpA patients, while qRT-PCR analysisudconfirmed data universality and specificity. ----- Conclusion: Our findings showed that jSpA is polygenic disease with malfunction in antigenudrecognition and activation of immunological response, in migration of inflammatory cells andudin regulation of the immune system. Results of our study can be used for further investigationudof disease patophisiology and new treatment options.