Intratumorska heterogenost izraženosti citokina CXCR4 i CXCL12 i njihova prognostička vrijednost u planocelularnim karcinomima grkljana Intratumoral heterogeneity of CXCR4 and CXCL12 expression and their prognostical value in laryngeal squamous cell carcinoma

摘要

The study includes 90 patients operated on squamous cell laryngeal carcinoma, who all had total laryngectomy, without preoperative radiation or chemotherapy. The results show that laryngeal squamous cell carcinoma express nuclear and cytoplasmatic expression of CXCR4, but nuclear expression is more prominent. The nuclear CXCR4 and CXCL12 expression is different in three different tumoral zones (transformational region, center of the tumor and the invasive margin). The lowest expression of CXCR4 and CXCL12 was in the center of the tumor, and the highest in the transformational region and in the invasive margin for CXCR4 and CXCL12, respectively. The correlation of expression of CXCR4 and CXCL12 with classical prognostic parameters (TNM classification, histological gradus and localization) is not statistically significant, except the correlation between low CXCL12 expression in the center of the tumor and histological gradus 1. Rpart statistical system got three times higher relative risk of death in the patients with positive expression of nuclear CXCR4 in the invasive margin of the tumor, in relation to CXCR4 negative tumors. Relative risk of death in the patients with highest CXCL12 positivity in the invasive margin of the tumor is two times decreased. Additional investigations are needed to confirm this work.