Human skeletal muscle pyruvate dehydrogenase phosphatase activity and expression : the effect of aerobic capacity

摘要

Activation of pyruvate dehydrogenase (PDH), which converts pyruvate into acetyl-CoA,is accomplished by a pair of specific phosphatases (PDP 1 & 2). A cross-sectional studyinvestigating the effect of aerobic capacity on PDP activity and expression found that: 1)PDP activity and PDP! protein expression were positively correlated with most aerobiccapacity measures in males (n=lS), but not females (n=12); 2) only males showed apositive correlation between PDP activity and PDPl protein expression (r=0.47; p=O.05),indicating that the increase in PDP activity in males is largely explained by increasedPDPl protein expression, but that females rely on another level for PDP activityregulation; and 3) PDP} and Ela protein expression increase in unison when expressedrelative to the E2 core. These data suggest that with increased aerobic capacity there isan increased capacity for carbohydrate oxidation through PDH, via El a, and an increasedability to activate PDH, via PDP, when exercising maximally.