UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN JERUK PURUT (Citrus hystrix D. C.) TERHADAP Pseudomonas aeruginosa DANudStaphylococcus epidermidis

摘要

Kaffir lime (Citrus hystrix D. C.) is a citrus plant that is generally used as a flavorudenhancer in food and beverages. This plant is native to Indonesia, which containsudflavonoids, alkaloids, saponins, steroids and tannins. This study aims to determine theudability of antibacterial extract lime leaves against Pseudomonas aeruginosa andudStaphylococcus epidermidis. Theresearch design was completely randomizedudtreatment with various concentrations of methanol extract lime leaves. The researchudbegins with drying leaves the oven at a temperature of 45 ˚C, pulverizing the meshud61, and extraction by maceration for 48 hours with methanol. This study uses audcompletely randomized design with treatments in variation concentrations of 12,5,ud25, and 50% concentration were then used to test the antibacterial activity. Inhibitionudzone formed from extracts with concentrations of 12,5, 25, and 50% againstudPseudomonas aeruginosa respectively were 0,605, 1,132, and 1,934 cm2, whereasudagainst Staphylococcus epidermidis produced successive inhibition zone were 0.518,ud0.837, and 1.251 cm2. Based on the research that has been done a concentration ofud50% was the concentration of the most effective in inhibiting the growth ofudStaphylococcus epidermidis and Pseudomonas aeruginosa. Then the smallestudconcentration of inhibitory zone, was 12,5% followed by the MIC test. Based on theudresults obtained by the MIC value for both types of bacteria is was 7,5%.