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Applications of immunocolloids in light microscopy. III. Demonstration of antigenic and lectin-binding sites in semithin resin sections

机译:免疫胶体在光学显微镜中的应用。三,在半薄树脂切片中证明抗原和凝集素结合位点

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摘要

Previous studies have demonstrated that antigens or lectin-binding sites can be localized in sections from paraffin-embedded tissues with protein A or lectins bound to colloidal gold or colloidal silver (Roth J: J Histochem Cytochem 30:691, 1982 and 31:547, 1983). In the present study the protein A-gold technique and lectin-gold complexes have been applied to semithin sections (0.5-1.5 micron) of Epon- or low temperature Lowicryl K4M-embedded rat pancreas, kidney and submandibular gland. The results show that an increase in resolution and, therefore, in amount of information can be obtained. The optimal mode of imaging was determined on sections without counterstaining. Bright-field illumination gives the maximum information about the staining signal, while phase-contrast and Nomarski differential interference contrast give predominantly structural and, to a lesser extent, staining information. Polarization epi- and transillumination microscopy is inferior in all aspects. The application of a battery of lectin-gold complexes to rat submandibular gland revealed a specific staining pattern for each lectin in acinar and excretory duct cells.
机译:先前的研究表明,抗原或凝集素结合位点可以定位在石蜡包埋组织的切片中,该组织的蛋白A或凝集素与胶体金或胶体银结合(Roth J:J Histochem Cytochem 30:691,1982和31:547, 1983)。在本研究中,已将蛋白A-金技术和凝集素-金复合物应用于埋有Epon或低温Lowicryl K4M的大鼠胰腺,肾脏和下颌下腺的半薄切片(0.5-1.5微米)。结果表明,可以获得分辨率的提高,因此可以获得信息量的提高。在没有复染色的情况下确定了最佳成像模式。明场照明可提供有关染色信号的最大信息,而相衬和Nomarski微分干涉对比则主要提供结构信息,在较小程度上提供染色信息。偏振镜和透照显微镜在所有方面均次于。将一组凝集素-金复合物应用于大鼠下颌下腺揭示了腺泡和排泄导管细胞中每种凝集素的特定染色模式。

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  • 作者

    Lucocq, J M; Roth, J;

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  • 年度 1984
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  • 原文格式 PDF
  • 正文语种 eng
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