Determination of fluoride in water by reversed-phase high-performance liquid chromatography using F--La3+- alizarin complexone ternary complex

摘要

A method for the determination of fluoride by reversed-pkase, high-performance liquid chromatography (RP-HPLC) is described. Fluoride, La 3, and alizarin complexone form F-La3+- alizarin complexone ternary complex, which is separated from the matrix on a RP, Ultrasphere C-18 column (250 x 4.6 mm, 5 mum) using methanol-water (19:81, v/v) mobile phase at 1.00 mL min(-1) - detection at 568 nm. The calibration graph was linear from 1.0-150 ng mL(-1) for fluoride with a correlation coefficient: 0.9993 (n = 6). The detection limit was 0.2 ng mL-1. The method was successfully applied to the determination of fluoride in river and tap water. Recovery was: 94-102%, RSD in the range: 1.9-3.6%.