BACKGROUNDudInactivation processes can be classified into non-thermal inactivation methods such as ethylene oxide and γ-radiation, and thermal methods such as autoclaving. The ability of carbon dioxide enriched microbubbles to inactivate Pseudomonas putida suspended in physiological saline, as a non-thermal sterilisation method, was investigated in this study with many operational advantages over both traditional thermal and non-thermal sterilisation methods.ududRESULTSudIntroducing carbon dioxide enriched microbubbles can achieve ∼2-Log reduction in the bacterial population after 90 min of treatment, addition of ethanol to the inactivation solution further enhanced the inactivation process to achieve 3, 2.5 and 3.5-Log reduction for 2%, 5% and 10 %( v/v) ethanol, respectively. A range of morphological changes was observed on Pseudomonas cells after each treatment, and these changes extended from changing cell shape from rod shape to coccus shape to severe lesions and cell death. Pseudomonas putida KT 2440 was used as a model of gram-negative bacteria.ududCONCLUSIONudUsing CO2 enriched microbubbles technology has many advantages such as efficient energy consumption (no heat source), avoidance of toxic and corrosive reagents, and in situ treatment. In addition, many findings from this study could apply to other gram-negative bacteria.
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机译:背景技术灭活过程可分为非热灭活方法,例如环氧乙烷和γ辐射,以及热灭活方法,例如高压灭菌。在这项研究中,研究了富含二氧化碳的微泡灭活悬浮在生理盐水中的恶臭假单胞菌的能力,这是一种非热灭菌方法,与传统的热灭菌和非热灭菌方法相比,具有许多操作优势。 ud udRESULTS ud经过90分钟的处理后,富含二氧化碳的微气泡可以使细菌种群减少约2-Log,将乙醇添加到灭活溶液中进一步增强了灭活过程,分别实现了3、2.5和3.5-Log减少,分别为2%,5%和分别为10%(v / v)乙醇。每次处理后,在假单胞菌细胞上观察到一系列形态学变化,这些变化从细胞形状从杆状变为球菌状延伸到严重损伤和细胞死亡。 ud ud结论 ud使用富含CO2的微泡技术具有许多优势,例如有效的能源消耗(无热源),避免使用有毒和腐蚀性的试剂以及就地处理等优点。此外,这项研究的许多发现可能适用于其他革兰氏阴性细菌。
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