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Reevaluation of the role of Pex1 and dynamin-related proteins in peroxisome membrane biogenesis

机译:重新评估pex1和发动蛋白相关蛋白在过氧化物酶体膜生物发生中的作用

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摘要

A recent model for peroxisome biogenesis postulates that peroxisomes form de novo continuously in wild-type cells byudheterotypic fusion of endoplasmic reticulum–derived vesicles containing distinct sets of peroxisomal membrane proteins.udThis model proposes a role in vesicle fusion for the Pex1/Pex6 complex, which has an established role in matrix proteinudimport. The growth and division model proposes that peroxisomes derive from existing peroxisomes. We tested theseudmodels by reexamining the role of Pex1/Pex6 and dynamin-related proteins in peroxisome biogenesis. We found thatudinduced depletion of Pex1 blocks the import of matrix proteins but does not affect membrane protein delivery to peroxisomes;udmarkers for the previously reported distinct vesicles colocalize in pex1 and pex6 cells; peroxisomes undergoudcontinued growth if ission is blocked. Our data are compatible with the established primary role of the Pex1/Pex6udcomplex in matrix protein import and show that peroxisomes in Saccharomyces cerevisiae multiply mainly byudgrowth and division.
机译:最近的过氧化物酶体生物合成模型推测,过氧化物酶体通过内质网衍生的囊泡的异型融合,在野生型细胞中从头连续形成,这些囊泡包含过氧化物酶体膜蛋白的不同集合。复合物,在基质蛋白 udimport中具有确定的作用。生长和分裂模型建议过氧化物酶体源自现有的过氧化物酶体。我们通过重新检查Pex1 / Pex6和动力蛋白相关蛋白在过氧化物酶体生物发生中的作用来测试这些 udmodel。我们发现诱导的Pex1耗竭阻止了基质蛋白的导入,但不影响过氧化物酶体的膜蛋白递送; 先前报道的不同囊泡的udmarkers共定位在pex1和pex6细胞中;如果阻滞,过氧化物酶体会持续生长。我们的数据与已确定的Pex1 / Pex6 udcomplex在基质蛋白输入中的主要作用是相容的,并表明酿酒酵母中的过氧化物酶体主要通过 udgrowth和除以繁殖。

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