A“Proteoglycan Targeting Strategy” for theudScintigraphic Imaging and Monitoring of the Swarm RatudChondrosarcoma Orthotopic Model

摘要

Our lab developed 99mTc-NTP 15-5 radiotracer as targeting proteoglycans (PGs) for the scintigraphic imaging of joint. This paperudreports preclinical results of 99mTc-NTP 15-5 imaging of an orthotopic model of Swarm rat chondrosarcoma (SRC). 99mTc-NTPud15-5 imaging of SRC-bearing and sham-operated animals was performed and quantified at regular intervals after surgery andudcompared to bone scintigraphy and tumoural volume. Tumours were characterized by histology and PG assay. SRC exhibiteduda significant 99mTc-NTP 15-5 uptake at very early stage after implant (with tumour/muscle ratio of 1.61 ± 0.14), whereas noudmeasurable tumour was evidenced. As tumour grew, mean tumour/muscle ratio was increased by 2.4, between the early and lateudstage of pathology. Bone scintigraphy failed to image chondrosarcoma, even at the later stage of study. 99mTc-NTP 15-5 imagingudprovided a suitable set of quantitative criteria for the in vivo characterization of chondrosarcoma behaviour in bone environment,uduseful for achieving a greater understanding of the pathology.