The development of novel instrumentation and analytical methodology forudquantitative microchip capillary electrophoresis (MCE) is described in thisudthesis. Demanding only small quantities of reagents and samples,udmicrofluidic instrumentation is highly advantageous. Fast separations at highudvoltages have been performed, which require the use of flexible high-voltageudpower supplies that have been developed in this work.udAn introductory overview of the general requirements of MCE is provided.udDifferences of chip-based CE and conventional CE are addressed, and theudeffects of buffer decomposition, reactions at electrodes, clogging ofudmicrochips, surface interactions, injection and power supply as well asuddetector-related problems on measurements are discussed. Additionally alsoudstrategies for proper analysis and technical solutions are identified.
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