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Signal-to-noise ratio enhancement of the compact light-emitting diode-induced fluorescence detector

机译:紧凑型发光二极管诱导荧光检测器的信噪比增强

摘要

To enhance the SNR (signal-to-noise ratio) of the compact light-emitting diode-induced fluorescence detector (LED-FD) ([14]), key parameters including LED and its coupling style, pinhole diameter, calibration, and refractive index matching fluid (RIMF) were optimized. A 20 mA LED with light intensity of 8400 mcd was used as new light source. To optimize pinhole diameter, a theoretical analysis based on a three-dimensional (3D) view of the detection area was proposed and validated experimentally. A calibration principle from the 3D perspective was proposed. The detection flow cell and the collection fiber were adjusted to be coplanar. RIMF (glycerol) was applied to enhance SNR. The performance of the improved LED-FD was evaluated by flow injection analysis (HA). The limit of detection (LOD) was determined as 0.15 nM sodium fluorescein (SNR=3). Compared with our previous work, a five-fold enhancement on the SNR was obtained. The LOD was about five times higher than that of commercial Agilent G1321A FLD which employed a xenon pulse lamp. (C) 2012 Elsevier B.V. All rights reserved.
机译:为了提高紧凑型发光二极管感应荧光检测器(LED-FD)的SNR(信噪比)([14]),关键参数包括LED及其耦合方式,针孔直径,校准和折射优化了指数匹配液(RIMF)。新的光源是20 mA LED,其光强度为8400 mcd。为了优化针孔直径,提出了基于检测区域的三维(3D)视图的理论分析,并进行了实验验证。从3D角度提出了校准原理。将检测流通池和收集纤维调整为共面。 RIMF(甘油)用于增强SNR。通过流动注射分析(HA)评估了改进的LED-FD的性能。检出限(LOD)确定为0.15 nM荧光素钠(SNR = 3)。与我们以前的工作相比,信噪比提高了五倍。 LOD大约是采用氙气脉冲灯的商业Agilent G1321A FLD的LOD的五倍。 (C)2012 Elsevier B.V.保留所有权利。

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