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Comprehensive gene expression analysis of 5'-end of mRNA identified novel intronic transcripts associated with hepatocellular carcinoma

机译:Comprehensive gene expression analysis of 5'-end of mRNa identified novel intronic transcripts associated with hepatocellular carcinoma

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摘要

To elucidate the molecular feature of human hepatocellular carcinoma (HCC), we performed 5'-end serial analysis of gene expression (5'SAGE), which allows genome-wide identification of transcription start sites in addition to quantification of mRNA transcripts. Three 5'SAGE libraries were generated from normal human liver (NL), non-B, non-C HCC tumor (T), and background non-tumor tissues (NT). We obtained 226,834 tags from these libraries and mapped them to the genomic sequences of a total of 8,410 genes using RefSeq database. We identified several novel transcripts specifically expressed in HCC including those mapped to the intronic regions. Among them, we confirmed the transcripts initiated from the introns of a gene encoding acyl-coenzyme A oxidase 2 (. ACOX2). The expression of these transcript variants were up-regulated in HCC and showed a different pattern compared with that of ordinary ACOX2 mRNA. The present results indicate that the transcription initiation of a subset of genes may be distinctively altered in HCC, which may suggest the utility of intronic RNAs as surrogate tumor markers. © 2010 Elsevier Inc.
机译:为了阐明人类肝细胞癌(HCC)的分子特征,我们进行了基因表达的5'-端序列分析(5'SAGE),除了对mRNA转录物进行定量分析外,它还允许全基因组范围内识别转录起始位点。从正常人肝脏(NL),非B,非C HCC肿瘤(T)和背景非肿瘤组织(NT)生成了三个5'SAGE文库。我们从这些库中获得226,834个标签,并使用RefSeq数据库将它们映射到总共8,410个基因的基因组序列。我们鉴定了几种在肝癌中特异性表达的新颖转录本,包括那些映射到内含子区域的转录本。其中,我们确认了从编码酰基辅酶A氧化酶2(。ACOX2)的基因的内含子开始的转录本。与普通ACOX2 mRNA相比,这些转录变体的表达在HCC中上调,并显示出不同的模式。目前的结果表明,基因的一个子集的转录起始可能在肝癌中显着改​​变,这可能提示内含子RNA可以作为替代肿瘤标志物。 ©2010爱思唯尔公司。

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