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A high-troughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)

机译：针对引起严重急性呼吸综合征（saRs）的人类病毒的高通量诊断分析

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The present invention relates to a diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (''hSARS virus''). In particular, the invention relates to a real-time quantitative PCR assay for the detection of hSARS virus using reverse transcription and polymerase chain reaction. Specifically, the quantitative assay is a TaqMan(R) assay using the primers and probes constructed based on the genome of the hSARS virus. The invention further relates to a diagnostic kit that comprises nucleic acid molecules for the detection of the hSARS virus.

机译：本发明涉及对人中引起严重急性呼吸道综合症（SARS）的病毒（“ hSARS病毒”）的诊断测定。特别地，本发明涉及用于使用逆转录和聚合酶链反应检测hSARS病毒的实时定量PCR测定法。具体地说，定量测定是使用基于hSARS病毒基因组构建的引物和探针的TaqMan测定。本发明进一步涉及一种诊断试剂盒，其包含用于检测hSARS病毒的核酸分子。

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1. Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses. [J] . Hadjinicolaou AV, Farcas GA, Demetriou VL, Archives of virology . 2011,第4期

机译：基于分子信标的多等位基因实时RT-PCR检测技术的开发，用于检测引起严重急性呼吸系统综合症（SARS-CoV）的人类冠状病毒：一种用于检测快速变异病毒的通用方法。
2. Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses [J] . Andreas V. Hadjinicolaou, Gabriella A. Farcas, Victoria L. Demetriou, Archives of Virology . 2011,第4期

机译：基于分子信标的多等位基因实时RT-PCR检测方法的开发，用于检测引起严重急性呼吸综合征的人冠状病毒：SARS-CoV：一种检测快速变异病毒的通用方法
3. Differential Sensitivities of Severe Acute Respiratory Syndrome (SARS) Coronavirus Spike Polypeptide Enzyme-Linked Immunosorbent Assay (ELISA) and SARS Coronavirus Nucleocapsid Protein ELISA for Serodiagnosis of SARS Coronavirus Pneumonia [J] . Patrick C. Y. Woo, Susanna K. P. Lau, Beatrice H. L. Wong, Journal of Clinical Microbiology . 2005,第7期

机译：严重急性呼吸系统综合症（SARS）冠状病毒穗状多肽酶联免疫吸附测定（ELISA）和SARS冠状病毒核衣夹蛋白ELISA对SARS冠状病毒肺炎的血清学诊断的敏感性差异
4. Severe Acute Respiratory Syndrome (SARS) Development of Diagnostics and Antivirals [C] . MORTEN DRAEBY SORENSEN, BRIAN SORENSEN, REGINA GONZALEZ-DOSAL, International Association of Biomedical Gerontology International . 2006

机译：严重急性呼吸综合征（SARS）开发诊断和抗病毒
5. Molecular biology of the Severe Acute Respiratory Syndrome coronavirus (SARS -CoV) accessory proteins ORF7a and ORF7b [D] . Schaecher, Scott Richard 2008

机译：严重急性呼吸系统综合症冠状病毒（SARS -CoV）辅助蛋白ORF7a和ORF7b的分子生物学
6. Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses [O] . Andreas V. Hadjinicolaou, Gabriella A. Farcas, Victoria L. Demetriou, -1

机译：基于分子信标的多等位基因实时RT-PCR检测方法的开发用于检测引起严重急性呼吸综合征的人冠状病毒：SARS-CoV：一种检测快速变异病毒的通用方法
7. A high-troughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS) [O] . 2006

机译：针对引起严重急性呼吸综合征（saRs）的人类病毒的高通量诊断分析

A high-troughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)

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