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Surface and intracellular interleukin-2 receptor expression on various resting and activated populations involved in cell-mediated immunity in human peripheral blood

机译:表面和细胞内白细胞介素-2受体在涉及人外周血中细胞介导的免疫的各种静息和活化群体中的表达

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摘要

The kinetics of assembly of the high-affinity interleukin-2 receptor (IL-2R)alpha/beta/gamma were investigated by studying intracellular and surface expression of IL-2Ralpha, beta and gamma by T cells, monocytes and natural killer (NK) cells. IL-2Ralpha and IL-2Rgamma were expressed by small numbers of resting T cells. These numbers increased following stimulation, to maximal expression at 48 h and 72 h, respectively. This observation was consistent with de novo synthesis of the receptor protein in response to the stimulus. The proportion of T cells producing IL-2Rbeta was smaller and up-regulated later than the proportion of cells producing IL-2Ralpha or IL-2Rgamma. IL-2Rbeta may therefore slow the assembly of the high-affinity IL-2R on T cells. A small number of resting NK cells expressed IL-2Ralpha, both on the cell surface and intracellularly, but this increased over 72 h on stimulated NK cells. IL-2Rbeta was constitutively expressed, both on the cell surface and intracellularly, by monocytes and NK cells. An increased proportion of NK cells and monocytes produced IL-2Rbeta, 24 h and 4 h post-stimulation, respectively. Maximal or plateau expression occurred at 72 h and 24 h post-stimulation, for NK cells and monocytes, respectively. The early up-regulation of intracellular IL-2Rbeta for monocytes may facilitate the up-regulation of surface IL-2Rbeta, and early assembly of the high-affinity IL-2R, accelerating monocyte activation and function. High constitutive intracellular IL-2Rgamma expression (> 80%) in all types of leucocyte investigated, decreased over the 72 h following stimulation with a concurrent increase in surface expression. IL-2Rgamma was expressed by increased proportions of T cells, monocytes and NK cells, 4 h following stimulation. The intracellular storage of IL-2Rgamma may accelerate translocation to the cell surface after stimulation. The early translocation of IL-2Rgamma may reflect its usage as a signal transduction molecule by other cytokine receptors - IL-4, IL-7, IL-9 and IL-15. This study delineated the potential expression of the high-affinity IL-2Ralpha/beta/gamma on various stimulated leucocytes. The differential kinetics of assembly of the high-affinity IL-2Ralpha/beta/gamma on different leucocyte subsets suggests that IL-2 may regulate the inflammatory cellular responses in a sequential manner, paralleling the timed expression of IL-2Ralpha/beta/gamma on the monocytes, NK cells and T cells.
机译:通过研究T细胞,单核细胞和自然杀伤分子(NK)在细胞内和表面表达IL-2Ralpha,β和γ来研究高亲和性白介素2受体(IL-2R)α/β/γ的组装动力学。细胞。 IL-2Ralpha和IL-2Rgamma由少量静止的T细胞表达。这些数目在刺激后增加,分别在48小时和72小时达到最大表达。该观察结果与响应刺激而从头合成受体蛋白一致。产生IL-2Rbeta的T细胞比例比产生IL-2Ralpha或IL-2Rgamma的细胞比例更小,上调时间更晚。因此,IL-2Rbeta可能会减慢高亲和力IL-2R在T细胞上的装配。少数静止的NK细胞在细胞表面和细胞内均表达IL-2Ralpha,但在受刺激的NK细胞上增加了72小时。 IL-2Rβ通过单核细胞和NK细胞在细胞表面和细胞内组成性表达。分别在刺激后24小时和4小时,增加比例的NK细胞和单核细胞产生IL-2Rbeta。 NK细胞和单核细胞分别在刺激后72 h和24 h达到最大或平台表达。早期细胞内IL-2Rbeta对单核细胞的上调可能促进表面IL-2Rbeta的上调,并促进高亲和力IL-2R的早期装配,从而加速单核细胞的激活和功能。在所研究的所有白细胞中,高组成型细胞内IL-2Rγ表达(> 80%)在刺激后72小时内下降,同时表面表达同时增加。刺激后4小时,T细胞,单核细胞和NK细胞的比例增加,从而表达IL-2Rγ。 IL-2Rγ的细胞内储存可能会刺激后加速向细胞表面的转运。 IL-2Rγ的早期易位可能反映出它被其他细胞因子受体-IL-4,IL-7,IL-9和IL-15用作信号转导分子。这项研究描述了高亲和力IL-2Ralpha / beta / gamma在各种刺激的白细胞上的潜在表达。高亲和力IL-2Ralpha / beta / gamma在不同白细胞亚群上组装的动力学差异表明,IL-2可能以顺序方式调节炎症细胞反应,与IL-2Ralpha / beta / gamma的定时表达平行单核细胞,NK细胞和T细胞。

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