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Acoustophoretic microfluidic chip for sequential elution of surface bound molecules from beads or cells

机译:声泳微流控芯片,用于从珠子或细胞中顺序洗脱表面结合的分子

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摘要

An acoustophoresis-based microfluidic flow-chip is presented as a novel platform to facilitate analysis of proteins and peptides loosely bound to the surface of beads or cells. The chip allows for direct removal of the background surrounding the beads or cells, followed by sequential treatment and collection of a sequence of up to five different buffer conditions. During this treatment, the beads/cells are retained in a single flow by acoustic radiation force. Eluted peptides are collected from the outlets and subsequently purified by miniaturized solid-phase extraction and analyzed with matrix assisted laser desorption mass spectrometry. Fundamental parameters such as the system fluidics and dispersion are presented. The device was successfully applied for wash and sequential elution of peptides bound to the surface of microbeads and human spermatozoa, respectively. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4749289]
机译:基于声泳的微流控芯片是一种新型平台,可帮助分析松散结合在珠子或细胞表面的蛋白质和多肽。该芯片可直接去除珠子或细胞周围的背景,然后依次处理并收集多达五个不同缓冲液条件的序列。在该处理过程中,珠粒/细胞通过声辐射力保持在单一流动中。从出口收集洗脱的肽,然后通过微型固相萃取进行纯化,并用基质辅助激光解吸质谱法进行分析。介绍了基本参数,例如系统射流和扩散。该设备已成功应用于分别清洗和顺序洗脱与微珠和人精子表面结合的肽。 (C)2012美国物理研究所。 [http://dx.doi.org/10.1063/1.4749289]

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