A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches

摘要

A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphicmicrobial communities is presented. In order to confirm compatibility with downstream analyses, bacterialT-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, withmetaproteomics undertaken on the corresponding protein fraction.