Tryptic cleavage of proteinaceous paint : a high-­performance protein binder analytical technique

摘要

This dissertation focuses on the development of novel, better performing protein binder analysis techniques inspired by contemporary proteomics whilst decreasing sample consumption. The analysis of peptides, a popular subject in proteomics, offers better specificity and a far improved ability to analyse blends of multiple proteins in comparison the established techniques for protein binders. Proteins are broken down into peptides by enzymatic processes. These peptides are small enough to be accurately measured, but large enough to hold the necessary specificity to unambiguously characterise their parent proteins. Potentially even small mutations between analogous proteins in different species can be distinguished this way. As a result of this high specificity, individual proteins in even the most complex mixtures would be easy to identify next to each other.