Real-time qPCR as a tool for evaluating RNAi-mediated gene silencing

机译：实时qpCR作为评估RNai介导的基因沉默的工具

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This article describes how rt-qPCR can be implemented as a tool to monitor silencing efficiency and functional effects of RNA interference (RNAi)-mediated gene knockdown, using examples from our research on neuroblastoma.

机译：本文使用来自神经母细胞瘤研究的实例，介绍了如何将rt-qPCR用作监测沉默的效率和RNA干扰（RNAi）介导的基因敲低的功能效应的工具。

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Van Maerken Tom; Mestdagh Pieter; De Clercq Sarah; Pattyn Filip; Yigit Nurten; De Paepe Anne; Marine Jean-Christophe; Speleman Franki; Vandesompele Jo;
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年度 2008
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1. Application of Real-Time qPCR Broadens: Technology's Ability to Quantify RNA Levels Makes It Chief among the Many Tools Used to Understand Gene Silencing [J] . Vicki Glaser Genetic Engineering & Biotechnology News: The Information Source of the Biotechnology Industry . 2007 ,第18期

机译：实时qPCR的应用拓宽了：技术定量RNA水平的能力使其成为了解基因沉默的众多工具中的佼佼者
2. Candidate reference genes for quantitative real-time PCR (qPCR) assays during development of a diet-related enteropathy in Atlantic salmon (Salmo salar L.) and the potential pitfalls of uncritical use of normalization software tools. [J] . Kortner T. M., Valen E. C., Kortner H., Aquaculture . 2011 ,第3a4期

机译：在大西洋鲑鱼（ Salmo salar ）的饮食相关性肠病发生期间进行定量实时PCR（qPCR）分析的候选参考基因，以及非严格使用标准化软件工具的潜在陷阱。
3. Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in three stingless bee species (Hymenoptera: Apidae: Meliponini) [J] . Flávia C. P. Freitas, Thiago S. Depintor, Lucas T. Agostini, Scientific reports. . 2019 ,第1期

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4. Evaluation of dqPCR Platform as a Liquid Biopsy ctDNA Diagnostic Tool for NSCLC Patients [C] . Max Cheng-Wey Wei International Molecular Medicine Tri-Conference. . 2019

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5. RNAi-Mediated Gene Silencing in Bed Bugs [D] . Basnet, Sanjay. 2018

机译：RNAi介导的基因在床上臭虫沉默
6. RNAi-Mediated Specific Gene Silencing as a Tool for the Discovery of New Drug Targets in Giardia lamblia; Evaluation Using the NADH Oxidase Gene [O] . Jaime Marcial-Quino, Saúl Gómez-Manzo, Francisco Fierro, 2017

机译：RNAi介导的特异性基因沉默作为在贾第鞭毛虫中发现新药靶标的工具；使用NADH氧化酶基因进行评估
7. Using real-time qPCR to evaluate RNAi-mediated gene silencing [O] . Van Maerken Tom, Mestdagh Pieter, De Clercq S, 2009

机译：使用实时qpCR评估RNai介导的基因沉默

Real-time qPCR as a tool for evaluating RNAi-mediated gene silencing

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