Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes

摘要

Gram-negative, rod-shaped bacteria were isolated from Lupinus polyphyllus, Lathyrus latifolius and Robinia pseudoacacia root nodules. Based on the 16S rRNA gene phylogeny, they were closely related to Bosea species (100 - 97%), belonging to the Alphaproteobacteria class and Bradyrhizobiaceae family. The closest relative for LMG 26383T, LMG 26379T and LMG 26381T were respectively, Bosea thiooxidans (99.6%), Bosea eneae (98.3%) and Bosea minatitlanensis (99.0%). Chemotaxonomic data, including the major fatty acid profiles supported the assignment of our strains to the genus Bosea. Analysis of the concatenated sequences of five housekeeping genes (atpD, dnaK, gyrB, recA and rpoB) and the results of DNA-DNA hybridisations, physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from each other and from the five validly published Bosea species. No nodA, nor nodC genes could be amplified, while nifH PCR gave non-specific products. On the basis of genotypic and phenotypic data three novel species, Bosea lupini sp. nov. (LMG 26383T = CCUG 61248 T), Bosea lathyri sp. nov. (= LMG 26379T= CCUG 61247 T) and Bosea robiniae sp. nov. (LMG 26381T= CCUG 61249 T) are proposed.