Improved solvent extraction procedure and HPLC-ELSD method for analysis of polar lipids from dairy materials

摘要

A normal phase HPLC-ELSD method which was improved from the method of Rombaut et al. 2005 (J. Dairy Sci. 88(2):482-488) for analysis of polar lipids (PLs) is presented. In the improved method, the mobile phase consisted of two lines; dichloromethane and a mixture of methanol and acetic acid/triethylamine buffer. Dichloromethane is less toxic than chloroform which was used in the old method. PLs of interest such as glycolipids, phospholipids and sphingomyelin were well separated with a total time for one analysis run of 22.5 min. Peak retention times and peak area were reproducible due to a good miscibility of mobile phases. The improved HPLC-ELSD method was applicable for both PLs from soy lecithin and dairy materials. Furthermore, a modified solvent extraction method of PLs from dairy matrices was adapted. The modified method offered higher extraction efficiency, consumed less time and in some cases saved solvent use.