The effect of chemical mutagen on haemolymph proteins of silkworm, Bombyx mori L.(Lepidoptera: Bombycidae) in F 1 stage

摘要

The chemical mutagen Diethylsulphate (DES) was treated to the mulberry silkworm, NB4D2 breed at the age, fifth instar fifth day with different concentrations (8mM and 10mM) by injection and spray methods. The F1 progeny was obtained from DES treated and control sets by selfing the moths. The quantitative analysis of haemolymph proteins revealed that 8mM injection set exhibit higher levels when compared with control and treated sets. The quantitative analysis also clearly indicate the variations in the number as well as intensity of the protein bands.