Effect of cooking on proteinase inhibitors of Dolichos lablab bean (Dolichos lablab purpureus L.)

摘要

Proteinase inhibitory activity in ten different varieties of Dolichos lablab purpureus was detd. All the varieties tested exhibited appreciable levels of proteinase inhibitory activity (PIA)​. The trypsin inhibitory activity (TIA) (Mean:20170 TIU​/g) was relatively higher than the chymotrypsin inhibitory activity (CIA) (Mean:15380 CIU​/g)​. Effect of temp. and cooking on PIA was studied. The nature of cooking medium and duration of cooking had profound effect on the PIA. The dry fried seeds lost their PIA very rapidly (91​% in 20 min)​. Seeds cooked in slightly alk. medium lost their PIA quickly (89​% in 30 min) compared to those cooked in acidic (80​% in 30 min) and neutral pH (83​% in 30 min)​. The PIA in green pods was also detd. and they had only one third of the PIA (8200 TIU​/g and 8125 CIU​/g) found in the dry seeds.