Regeneration of Shoots from Leaf Callus Cultures of Drymaria Cordata (l) Willd Ex Roem and Schult

摘要

An efficient protocol for rapid regeneration of shoots from leaf callus cultures of Drymaria cordata was developed. Creamish white profuse callus proliferation was obtained on MS supplemented with NAA (2.69ìM) + BAP (4.44ìM) after three weeks of culture. The callus became green, compact and nodular on transfer to TDZ (0.91ìM) supplemented medium. Shoot buds were initiated from the callus after 8 weeks of culture on the solid medium. The frequency of initiation of shoot buds could be enhanced if the green nodulated callus was transferred to filter paper placed on liquid MS medium supplemented with TDZ (2.27 – 4.54ìM). The shoots have developed roots on IBA (0.49ìM) supplemented MS solid medium. Thus obtained plantlets were successfully hardened and transferred to soil. Nearly 90% survival rate was recorded.