The application of immobilized lipase (EC 3.1.1.3) is gaining interest in the oleochemical industry as it offers advantages over conventional chemical reactions. In the present study, a commercial immobilized lipase, Lipozyme TL IM, was used to catalyze the hydrolysis of palm olein in an aqueous-organic phase. A ping-pong bi-bi model with substrate inhibition by water was used to describe the hydrolysis reaction. The reaction rate constants of the proposed mechanism were determined by fitting the model into experimental data using a nonlinear curve fitting software. Based on the results of this study, the model proposed was able to fit the data with a correlation coefficient of 0.9586. The rate of formation of fatty acids is limited by the formation of glycerol. The critical water content before inhibition occurs was found to be 3.6% (v/v). No inhibition by palm olein was observed up to a concentration of 874.76 g/l.
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机译：固定化脂肪酶（EC 3.1.1.3）的应用在油脂化学工业中引起了人们的兴趣，因为它具有优于常规化学反应的优势。在本研究中，商业化的固定化脂肪酶Lipozyme TL IM用于催化有机油相中棕榈油精的水解。使用具有水底物抑制作用的乒乓球bi-bi模型描述水解反应。通过使用非线性曲线拟合软件将模型拟合到实验数据中，可以确定所提出机制的反应速率常数。根据这项研究的结果，提出的模型能够拟合数据，相关系数为0.9586。脂肪酸的形成速率受甘油形成的限制。发现抑制发生之前的临界水含量为3.6％（v / v）。直至浓度为874.76 g / l，都未观察到棕榈油精的抑制作用。
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