Embryonic stem cells biosensor and its application in drug analysis and toxin detection

摘要

To investigate the use of stem cells as biosensor elements, a novel cell-based light-addressable Potentiometric sensor (LAPS) was developed for monitoring cellular beating. Mouse embryonic stem cells were induced to differentiate into cardiomyocytes in vitro. Extracellular field potentials of spontaneously beating cardiomyocytes induced from stem cells were recorded by LAPS in the potential and frequency ranges of 25-45 ?gV and 0.5-3 Hz, respectively. Due to its capability of monitoring important physiological parameters such as potential and frequency in vitro, the sensor can be used in drug analysis and toxin detection in a long-term and noninvasive way. The pharmacological and toxicological researches make it possible to use stem cells-based biosensor for biomedical assays.