Determination of permethrin and its metabolites in hearts and urines of rats by GC-ECD and GC-MS

摘要

Pyrethroids are pesticides very used in agriculture, which tend to replaceorganophosfate and carbamate insecticides. These pesticides have shown to exhibitcardiotoxicity.The aim of this study was to assess if cardiotoxicity is due to direct or indirect effects(metabolites) of permethrin on hearts. There were studies 8 rats; three of them weresacrificed after 24 of the end of treatment with permethrin and the other four weresacrificed after 14 days of the end of treatment. Afterwards hearts and urines werecollected. The amounts of permethrin and its main metabolite (3-PBA) wereevaluated on hearts and urines of female rats which were treated with permethrin andsacrificed the day after and 14 days after the treatment. Moreover has beenhighlighted the difference of amount of permethrin and its metabolite between ratssacrificed immediately at the end of treatment and those sacrificed after 14 days. Thestudy of permethrin was accomplished by liquid-liquid extraction and GC-ECD. Theevaluation of 3-PBA was performed by SPE procedure with 2-PBA as internalstandard and gas-chromatography GC-MS. The concentration of permethrin in heartsis basically the same in 24h and 14 days. The 3-PBA concentration in urinesdecreased 50 times from 24h to 14 days. In hearts the 3-PBA level also decrease butonly 2.24 times and a high variation of results were achieved in rats after 14 days.