Increase of protein content and production of lignocellulolytic enzymes by simultaneous solid-state fermentations of brewery, olive mill and winery wastes

摘要

Frequently, the agro-industrial wastes are used as animal feed, however many timesthese wastes have a poor nutritional quality, mainly they have a low protein content andtheir digestibility is hard. These disadvantages can be avoided by solid-statefermentation (SSF) of the wastes by filamentous fungi.Winery, brewery and olive mill wastes were used as solid substrates for the productionof lignocellulolytic enzymes and to increase the crude protein content of agro-industrialwastes.After selection of the fungus in previously study, it was performed an experimentaldesign to evaluate the benefits of using mixtures of wastes in different proportions assolid substrate in SSF by A. ibericus. The optimum substrate was a mixture of breweryspent grain and vine-shoot trimmings which achieved and increase the protein content(16.3 %), xylanase (89.33 U/g), cellulose (3.46 U/g) and -glucosidase (21.91 U/g)activities.Through this study, it was possible to conclude that the SSF by A. ibericus is a suitablebiotechnology process to increase the nutritional quality of agro-industrial wastes andto produce value-added products as enzymes in the same low-cost process.