Physicochemical surface properties of brewing yeast influencing their immobilization onto spent grains in a continuous reactor

摘要

Immobilization of brewing yeast onto a cellulosebased carrier obtained from spent grains, a brewing byproduct, by acid/base treatment has been studied in a continuously operating bubble-column reactor. The aimof this work was to study the mechanisms of brewing yeast immobilization onto spent grain particles through the information on physicochemical surface properties ofbrewing yeast and spent grain particles. Three mechanisms of brewing yeast immobilization onto spent grains carrier were proposed: cell-carrier adhesion, cell-cell attachment, and cell adsorption (accumulation) inside natural shelters (carrier’s surface roughness). The possibilityof stable cell-carrier adhesion regarding the free energy of interaction was proved and the relative importance of longrange forces (Derjaguin-Landau-Verwey-Overbeek theory)and interfacial free energies was discussed. As for the cell-cell attachment leading to a multilayer yeast immobilization, a physicochemical interaction through localizedhydrophobic regions on cell surface was hypothesized.However, neither flocculation nor chain formation mechanism can be excluded so far. The adsorption of brewing yeast inside sufficiently large crevices (pores) was documented with photomicrographs. A positive effect of higher dilution rate and increased hydrophobicity of base-treatedspent grains on the yeast immobilization rate has also been found.