A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

摘要

Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique.Cellulases were hydrolysed with papain and the binding domains were then separated from the digestedmixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. Thismethodology produced a yield of 85% of binding domains.