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Carotenoid analysis of Cassava genotypes roots (Manihot Esculenta Crantz) cultivated in Southern Brazil using chemometric tools

机译:使用化学计量学工具对巴西南部种植的木薯基因型根(Manihot Esculenta Crantz)进行类胡萝卜素分析

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摘要

Manihot esculenta roots rich in -carotene are an important staple food for populations with risk of vitamin A deficiency. Cassava genotypes with high pro-vitamin A activity have been identified as a strategy to reduce the prevalence of deficiency of this vitamin, In this study, the metabolomics characterization focusing on the carotenoid composition of ten cassava genotypes cultivated in southern Brazil by UV-visible scanning spectrophotometry and reverse phase-high performance liquid chromatography was performed. The data set was used for the construction of a descriptive model by chemometric analysis. The genotypes of yellow roots were clustered by the higher concentrations of cis--carotene and lutein. Inversely, cream roots genotypes were grouped precisely due to their lower concentrations of these pigments, as samples rich in lycopene differed among the studied genotypes. The analytical approach (UV-Vis, HPLC, and chemometrics) used showed to be efficient for understanding the chemodiversity of cassava genotypes, allowing to classify them according to important features for human health and nutrition.
机译:富含-胡萝卜素的Manihot esculenta根是维生素A缺乏风险人群的重要主食。具有高维生素A活性的木薯基因型已被确定为减少该维生素缺乏症患病率的策略。在这项研究中,代谢组学研究的重点是通过紫外线可见扫描法在巴西南部种植的十种木薯基因型的类胡萝卜素组成分光光度法和反相高效液相色谱法。该数据集用于通过化学计量分析构建描述性模型。黄根的基因型被较高浓度的顺式-胡萝卜素和叶黄素聚类。相反,由于这些色素的浓度较低,因此霜根基因型被精确分组,因为富含番茄红素的样品在所研究的基因型中有所不同。所使用的分析方法(UV-Vis,HPLC和化学计量学)被证明对理解木薯基因型的化学多样性是有效的,从而可以根据对人类健康和营养的重要特征对其进行分类。

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