Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column

摘要

In this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentativebroth was proposed using a single activated charcoal column. The FOS adsorption onto the activatedcharcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25 8C.Mixtures containing 50.6% (w/w) of FOS (FOS content in the fermentative broth) were purified to 92.9%(w/w) with a FOS recovery of 74.5% (w/w). Moreover, with the proposed process, fractions with purity upto 97% (w/w) of FOS were obtained. This purification process was also found to be efficient in thedesalting of the fermentative broth.