Treatment of effluents containing phenols such as textile dyes with fungal laccases is usually limited to the acid to neutral pH range and moderate temperatures. Here we demonstrate for the first time that spore-bound laccases which are stable at high temperatures and pH values can be used for phenolic dye decolorisation. Laccase containing spores from Bacillus SF were immobilized on alumina pellets. Both immobilized and free spores were able tocompletely decolorize the common textile dyes Mordant Black 9, Mordant Brown 96/Mordant Brown 15, and Acid Blue 74 within 90 min of incubation time and decolorized solutions were successfully used in re-dyeing.spores from Bacillus SF were immobilized on alumina pellets. Both immobilized and free spores were able to completely decolorize the common textile dyes Mordant Black 9, Mordant Brown 96/Mordant Brown 15, and Acid Blue 74 within 90 min of incubation time and decolorized solutions were successfully used in re-dyeing.
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机译:用真菌漆酶处理含酚的废水(如纺织染料)通常限于酸至中性pH范围和中等温度。在这里,我们首次证明在高温和pH值下稳定的与孢子结合的漆酶可用于酚类染料的脱色。将来自芽孢杆菌SF的含有漆酶的孢子固定在氧化铝颗粒上。固定的和游离的孢子均能够在90分钟的孵育时间内完全使普通的纺织染料Mordant Black 9,Mordant Brown 96 / Mordant Brown 15和Acid Blue 74脱色,脱色溶液已成功用于Bacillus SF的再染色。将其固定在氧化铝颗粒上。固定孢子和游离孢子均能够在90分钟的孵育时间内使普通的纺织染料媒染剂Black 9,媒染剂Brown 96 /媒染剂Brown 15和酸性蓝74完全脱色,并且脱色溶液已成功用于重新染色。
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