Chitosan membranes containing micro or nano-size bioactive glass particles : evolution of biomineralization followed by in-situ dynamic mechanical analysis

摘要

A newfamilyofbiodegradablepolymer/bioactiveglass(BG)compositematerialshasemergedbasedontheavailabilityofnano-sizedbioactiveparticles.Suchnovelbiocompo-sites canhaveenhancedperformance,intermsofmechanicalpropertiesandbioactivity,and theycanbedesignedtobeusedinboneregenerationapproaches.In thiswork,membranesofchitosan(CTS)andchitosanwithbioactiveglass(BG)bothmicron andnanosizedparticles(CTS/mBG,CTS/nBG,respectively)werepreparedbysolvent casting.Microstructuralandmechanicalpropertieswereevaluatedinordertocompare theeffectsoftheincorporationofmicro(mBG) andnano(nBG)particlesinthechitosan matrix. In vitro bioactivity testswereperformedtocharacterizetheapatitelayerthat isformedonthesurfaceofthematerialafterbeingimmersedinsimulatedbodyfluid(SBF). Thebiomineralizationprocessonthebiomaterialswasalsofollowedusingnon-conventionaldynamicmechanicalanalysis(DMA),bothonlineandoffline.InsuchDMAexperiments,thechangeinthestoragemodulus, E0, andthelossfactor,tan d, weremeasured asafunctionoftheimmersiontimeinSBF.TheresultsdemonstratedthatCTS/nBG membranespossessenhancedmechanicalpropertiesandhigherbioactivityincomparisonwiththeCTS/mBG membranes.SuchresultssuggestthepotentialofnBGforthe developmentofbioactivecompositesforboneregenerationapplications.