Three-dimensional distribution of GFP-labeled Pseudomonas putida during biofilm formation on solid PAHs assessed by confocal laser scanning microscopy

摘要

Confocal laser scanning microscopy was used to monitor the colonization pattern of the gfplabeledderivative strain of Pseudomonas putida ATCC 17514 on fluorene and phenanthrene crystals. Thein situ experiments showed that P. putida tends to grow directly on phenanthrene, forming a biofilm onaccessible crystalline surfaces. On the other hand, no significant biofilm formation was observed in thepresence of fluorene. The results obtained showed that substrate properties affected bacterial strategyregarding uptake.