Modulating bone cells response onto starch-based biomaterials by surface plasma treatment and protein adsorption

摘要

The effect of oxygen-based radio frequency glow discharge (rfGD) on the surface of different starch-based biomaterials (SBB) and theinfluence of proteins adsorption on modulating bone–cells behavior was studied. Bovine serum albumin, fibronectin and vitronectin wereused in single and complex protein systems. RfGD-treated surfaces showed to increase in hydrophilicity and surface energy whencompared to non-modified SBB. Biodegradable polymeric blends of cornstarch with cellulose acetate (SCA; 50/50 wt%), ethylene vinylalcohol (SEVA-C; 50/50 wt%) and polycaprolactone (SPCL; 30/70 wt%) were studied. SCA and SCA reinforced with 10%hydroxyapatite (HA) showed the highest degree of modification as result of the rfGD treatment. Protein and control solutions wereused to incubate with the characterized SBB and, following this, MG63 osteoblast-like osteosarcoma cells were seeded over the surfaces.Cell adhesion and proliferation onto SCA was found to be enhanced for non-treated surfaces and on SCA+10%HA no alteration wasbrought up by the plasma modification. Onto SCA surfaces, BSA, FN and VN single solutions improved cell adhesion, and this sameeffect was found upscaled for ternary systems. In addition, plasma treated SEVA-C directed an increase in both adhesion andproliferation comparing to non-treated surfaces. Even though adhesion onto treated and untreated SPCL was quite similar, plasmamodification clearly promoted MG63 cells proliferation. Regarding MG63 cells morphology it was shown that onto SEVA-C surfacesthe variation of cell shape was primarily defined by the protein system, while onto SPCL it was mainly affected by the plasma treatment.