In this study, bilayered silk and silk/nano-CaP scaffolds were developed forosteochondral (OC) tissue engineering. Aqueous silk solution (16 wt.%) was used for preparation ofthe cartilage-like layer and, for generation of the silk/nano-CaP suspension and the bottom layer(CaP/Silk: 16 wt.%). The scaffolds were formed by using salt-leaching/lyophilization approach. Thescanning electron microscopy revealed that the both layers presented porous structure andintegrated well. Micro-computed tomography images confirmed that the CaP phase was onlyretained in the silk/nano-CaP layer. The hydration degree and mechanical properties of thebilayered scaffold were comparable to the ones of each single layer. The apatite crystal formationwas limited to the silk/nano-CaP layer, when soaking the scaffold in a simulated body fluidsolution, which is a must for the application of the developed scaffolds in OC tissue engineering
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