Three different media—Columbia agar, Wilkins-Chalgren agar, and Helicobacter pylori special peptoneagar—were prepared in a diluted version and compared to the standard medium formulation in order to studya possible nutrient shock effect observed when recovering H. pylori from water by counting the number of CFU.This same parameter was subsequently used to evaluate the influence of the incubation atmosphere by usinga modular atmosphere-controlled system to provide different atmospheres and by employing an established gasgeneration kit as a control. Both a low nutrient content of the media and a rapidly achieved microaerophilicincubation atmosphere proved to increase the numbers of environment-stressed H. pylori organisms recovered.An atmosphere of 5% CO2, 5% O2, and 3% H2 is recommended, although other atmospheres with a low oxygenconcentration are also acceptable. Besides highlighting and assessing the importance of several factors in theculturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique forrecovery of this pathogen from water.
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