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Degradation of azo dyes by trametes villosa laccase over long periods of oxidative conditions

机译:短绒毛球菌漆酶在长期氧化条件下降解偶氮染料

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摘要

Trametes villosa laccase was used for direct azo dye degradation, and the reaction products that accumulatedafter 72 h of incubation were analyzed. Liquid chromatography-mass spectrometry (LC-MS) analysis showedthe formation of phenolic compounds during the dye oxidation process as well as a large amount of polymerizedproducts that retain azo group integrity. The amino-phenol reactions were also investigated by 13C-nuclearmagnetic resonance and LC-MS analysis, and the polymerization character of laccase was shown. This studyhighlights the fact that laccases polymerize the reaction products obtained during long-term batch decolorizationprocesses with azo dyes. These polymerized products provide unacceptable color levels in effluents,limiting the application of laccases as bioremediation agents.
机译:用Trametes villosa漆酶直接降解偶氮染料,并分析了孵育72小时后积累的反应产物。液相色谱-质谱(LC-MS)分析表明,在染料氧化过程中酚类化合物的形成以及大量保留偶氮基团完整性的聚合产物。还通过13 C-核磁共振和LC-MS分析研究了氨基苯酚反应,并显示了漆酶的聚合特性。这项研究突出了漆酶将长期批量脱色过程中获得的反应产物与偶氮染料聚合的事实。这些聚合产物在废水中提供了不可接受的颜色水平,限制了漆酶作为生物修复剂的应用。

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