Application of wheat straw autohydrolysis liquor to xylanase and β-xylosidase large-scale production in a stirred tank bioreactor

摘要

Research into microbial xylanases production has increased due to its several applications.In this context, studies that make this practice feasible are important. Wheat bran is aninexpensive byproduct, which contains around 28% hemicellulose; however, the wheat branparticles suspended in the cultivation medium have to be decomposed to soluble compoundsto be used by the fungi and the treatment of lignocellulosic materials in autohydrolysisprocesses makes this easier. The inclusion of these treated materials in the nutrient media canbe a strategy to increase and undervalue xylanase production. The best conditions forxylanase and β-xylosidase production were observed when A. ochraceus was cultivated with1% wheat bran added 10% wheat straw autohydrolysis liquor as carbon source, thissubstrate was more favorable when compared with xylan, wheat bran and wheat strawautohydrolysis liquor used separately. The application of this substrate in a stirred tankbioreactor shows the need for improvements of the fermentation process.