Simple and selective spectrophotometric methods for the determination of domperidone in pharmaceuticals through charge transfer complex formation reaction.

摘要

Two simple, rapid and selective spectrophotometric methods are described for the detn. of domperidone (DOM) in pure drug and in pharmaceuticals. The methods are based on the formation of charge-transfer complex between DOM as n-donor and π-acceptors like 2, 3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in method A or p-chloranilic acid (p-CA) in method B. The products exhibit absorption maxima at 590 and 520 nm in acetone for method A and method B, resp. Under the optimum reaction conditions, linear relationships with good correlation coeffs. (0.998 in method A and 0.999 in method B) were found between the absorbance and the concn. of DOM in the ranges of 5-80 and 20-320 μg/mL in method A and method B, resp. The apparent molar absorptivity values are calcd. to be 4.88 × 103 and 1.14 × 103, L/mol/cm, for method A and method B, resp., with corresponding Sandell sensitivity values of 0.021 and 0.017 μg/cm2. The limit of detection (LOD) values are found to be 0.28 and 1.19 μg/mL for method A and method B, resp., with corresponding limit of quantification (LOQ) values of 0.86 and 3.59 μg/mL. The stoichiometry of the reaction was found to be 1:1 in both the cases. The proposed methods were applied successfully for the detn. of DOM in tablets with good accuracy and precision. The results obtained by the proposed methods were compared favorably with those of the ref. method. [on SciFinder(R)]