Development and validation of a normal phase HPLC method for separation udof anacardic acid isomers in cashew nut shell liquid

摘要

A simple normal phase HPLC method was developed and validated for the separation and determination of udanacardic acid isomers present in cashew nut shell liquid. A Chiralpack, IA Chiral column (250 mm x 4.6 mm, udpacked with 5 microns) is used as stationary phase. An isocratic mode with mobile phase consists of 0.1% udtrifluroacetic acid in n- Hexane and IPA in ration of 1:1000:10 (v/v/v), at a flow rate of 1 mL/min. and the eluent udwas monitored at a wavelength of 210 nm. The result obtained from method validation showed a good agreement udwith the declared content. The method shows good linearity in the range of 0.06-0.24 mg/mL with correlation udcoefficient 0.9997-0.9999. Analytical recovery was between 99.0 -102.0 %. The proposed method successfully udapplied for separation of anacardic acid isomers i.e. saturated, monoene, diene and triene present in cashew nut udshell liquid. The established normal phase HPLC method is precise, accurate and selective, because of its sensitivity udand reproducibility