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Microrheology of non mulberry silk varieties by optical tweezer and video microscopy based techniques

机译:非桑蚕丝品种的光流变学和光学显微镜技术

摘要

We have carried out a comparative study of the microrheological properties of silk fibroin solutions formed from a variety of silks indigenous to the Indian subcontinent. We present the measured viscoelastic moduli of Tasar silk fibroin solution using both a single and dual optical tweezer at 0.16% and 0.25% (w/v). The bandwidth of the measurements carried out using optical tweezers is extended down to the lower frequency regime by a video microscopy measurement. Further, we have measured the viscoelastic moduli of Eri and Muga varieties of silk fibroin solutions at a higher concentration (1.00% w/v) limiting the tool of measurement to video microscopy, as the reduced optical transparencies of these solutions at higher concentration preclude an optical tweezer based investigation. The choice of a higher concentration of fibroin solution of the latter silk varieties is so as to enable a comparison of the shear moduli obtainedudfrom optical methods with their corresponding fibre stiffness obtained from wide angle X-rayudscattering data. We report a correlation between the microstructure and microrheological parameters of these silk varieties for the concentration of fibroin solutions studied
机译:我们已经对由印度次大陆固有的多种丝形成的丝素蛋白溶液的微流变特性进行了比较研究。我们介绍了使用0.16%和0.25%(w / v)的单光和双光镊子测量的Tasar丝素蛋白溶液的粘弹性模量。使用光学镊子进行的测量带宽通过视频显微镜测量向下扩展至低频范围。此外,我们已经在较高浓度(1.00%w / v)下测量了丝纤蛋白溶液的Eri和Muga品种的粘弹性模量,将测量工具限制于视频显微镜,因为这些溶液在较高浓度下降低的光学透明性使得基于光学镊子的调查。后一种丝绸品种中较高浓度的丝蛋白溶液的选择是为了能够将光学方法获得的剪切模量与从广角X射线散射数据获得的相应纤维刚度进行比较。我们报告了这些丝的品种的微观结构和微观流变学参数之间的相关性,研究的纤维蛋白溶液的浓度

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