Spectrophotometric determination of zolmitriptan in bulk drug and pharmaceuticals using vanillin as a reagent.

摘要

An accurate and precise spectrophotometric method is presented for the detn. of zolmitriptan (ZMT) based on the formation of a red color product with vanillin in presence of concd. H2SO4, with the chromogen being measured at 580 nm. The reaction proceeds quant. at room temp. in 10 min. The calibration curve is linear over the range 5.0-90.0 μgmL-1 and described by the regression equation Y = (-)0.0101 + 0.0117X with a regression coeff. (r) of 0.9994 (n = 7). The calcd. molar absorptivity and Sandell sensitivity values are 3.3 × 103 Lmol-1cm-1 and 0.0872 μgcm-2, resp. The limits of detection (LOD) and quantification (LOQ) calcd. as per ICH guidelines are 1.26 and 3.81 μgmL-1, resp. The within-day accuracy expressed as relative error was better than 1.78% with precision (RSD) ranging from 0.83 to 1.45 %. The between-day accuracy ranged from 1.21 to 1.84 % with a precision less than 1.66%. The method was successfully applied to the anal. of one brand of tablet contg. zolmitriptan. The results obtained were in agreement with those obtained by published ref. method. The accuracy was also checked by placebo blank and synthetic mixt. analyses besides recovery study via std. addn. procedure. [on SciFinder(R)]